Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome.

TitleReactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome.
Publication TypeJournal Article
Year of Publication2006
AuthorsBrinkman, MBeth, McKevitt, M, McLoughlin, M, Perez, C, Howell, J, Weinstock, GM, Norris, SJ, Palzkill, T
JournalJ Clin Microbiol
Volume44
Issue3
Pagination888-91
Date Published2006 Mar
ISSN0095-1137
KeywordsAntibodies, Bacterial, Antigens, Bacterial, Bacterial Proteins, Enzyme-Linked Immunosorbent Assay, Humans, In Vitro Techniques, Protein Array Analysis, Proteome, Syphilis, Treponema pallidum
Abstract

To identify antigens important in the human immune response to syphilis, the serum antibody reactivity of syphilitic patients was examined with 908 of the 1,039 proteins in the proteome of Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay. Thirty-four proteins exhibited significant reactivity when assayed with human sera from patients in the early latent stage of syphilis. A subset of antigens identified were further scrutinized for antibody reactivity at primary, secondary, and latent disease stages, and the results demonstrate that the humoral immune response to individual T. pallidum proteins develops at different rates during the time course of infection.

DOI10.1128/JCM.44.3.888-891.2006
Alternate JournalJ. Clin. Microbiol.
PubMed ID16517872
PubMed Central IDPMC1393150
Grant ListR01 AI045842 / AI / NIAID NIH HHS / United States
R03 AI069107 / AI / NIAID NIH HHS / United States
AI45842 / AI / NIAID NIH HHS / United States
AI49557 / AI / NIAID NIH HHS / United States