Recognition of pore-forming colicin Y by its cognate immunity protein.

TitleRecognition of pore-forming colicin Y by its cognate immunity protein.
Publication TypeJournal Article
Year of Publication2006
AuthorsSmajs, D, Matejkov√°, P, Weinstock, GM
JournalFEMS Microbiol Lett
Volume258
Issue1
Pagination108-13
Date Published2006 May
ISSN0378-1097
KeywordsAmino Acid Sequence, Bacterial Proteins, Colicins, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Structure, Secondary, Structure-Activity Relationship
Abstract

Construction of hybrid immunity genes between colicin U (cui) and Y (cyi) immunity genes and site-directed mutagenesis of cyi were used to identify amino-acid residues of the colicin Y immunity protein (Cyi) involved in recognition of colicin Y. These amino-acid residues were localized close to the cytoplasmic site of the Cyi transmembrane helices T3 (S104, S107, F110, A112) and T4 (A159). Mutations in cui, which converted Cui sequence to Cyi sequence in positions 104, 107, 110, 112 and 159, resulted in an immunity gene that also conferred (besides immunity to colicin U) a high degree of immunity to colicin Y.

DOI10.1111/j.1574-6968.2006.00201.x
Alternate JournalFEMS Microbiol. Lett.
PubMed ID16630264