REGγ is associated with multiple oncogenic pathways in human cancers.

TitleREGγ is associated with multiple oncogenic pathways in human cancers.
Publication TypeJournal Article
Year of Publication2012
AuthorsHe, J, Cui, L, Zeng, Y, Wang, G, Zhou, P, Yang, Y, Ji, L, Zhao, Y, Chen, J, Wang, Z, Shi, T, Zhang, P, Chen, R, Li, X
JournalBMC Cancer
Date Published2012 Feb 23
KeywordsAntigens, Neoplasm, Autoantigens, Colonic Neoplasms, Disease Progression, Gene Expression Profiling, Humans, Immunohistochemistry, Liver Neoplasms, Lung Neoplasms, Microarray Analysis, Neoplasms, Proteasome Endopeptidase Complex, Thyroid Neoplasms, Tumor Cells, Cultured

BACKGROUND: Recent studies suggest a role of the proteasome activator, REGγ, in cancer progression. Since there are limited numbers of known REGγ targets, it is not known which cancers and pathways are associated with REGγ.METHODS: REGγ protein expressions in four different cancers were investigated by immunohistochemistry (IHC) analysis. Following NCBI Gene Expression Omnibus (GEO) database search, microarray platform validation, differential expressions of REGγ in corresponding cancers were statistically analyzed. Genes highly correlated with REGγ were defined based on Pearson's correlation coefficient. Functional links were estimated by Ingenuity Core analysis. Finally, validation was performed by RT-PCR analysis in established cancer cell lines and IHC in human colon cancer tissuesRESULTS: Here, we demonstrate overexpression of REGγ in four different cancer types by micro-tissue array analysis. Using meta-analysis of publicly available microarray databases and biological studies, we verified elevated REGγ gene expression in the four types of cancers and identified genes significantly correlated with REGγ expression, including genes in p53, Myc pathways, and multiple other cancer-related pathways. The predicted correlations were largely consistent with quantitative RT-PCR analysis.CONCLUSIONS: This study provides us novel insights in REGγ gene expression profiles and its link to multiple cancer-related pathways in cancers. Our results indicate potentially important pathogenic roles of REGγ in multiple cancer types and implicate REGγ as a putative cancer marker.

Alternate JournalBMC Cancer
PubMed ID22361172
PubMed Central IDPMC3350384
Grant List1R01CA131914 / CA / NCI NIH HHS / United States

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