Retinal organoids derived from rhesus macaque iPSCs undergo accelerated differentiation compared to human stem cells.

TitleRetinal organoids derived from rhesus macaque iPSCs undergo accelerated differentiation compared to human stem cells.
Publication TypeJournal Article
Year of Publication2022
AuthorsLopez, AJacobo, Kim, S, Qian, X, Rogers, J, J Stout, T, Thomasy, SM, La Torre, A, Chen, R, Moshiri, A
JournalCell Prolif
Date Published2022 Apr
KeywordsAnimals, Cell Differentiation, Humans, Induced Pluripotent Stem Cells, Macaca mulatta, Organoids, Pluripotent Stem Cells, Retina

PURPOSE: To compare the timing and efficiency of the development of Macaca mulatta, a nonhuman primate (NHP), induced pluripotent stem cell (rhiPSC) derived retinal organoids to those derived from human embryonic stem cells (hESCs).

RESULTS: Generation of retinal organoids was achieved from both human and several NHP pluripotent stem cell lines. All rhiPSC lines resulted in retinal differentiation with the formation of optic vesicle-like structures similar to what has been observed in hESC retinal organoids. NHP retinal organoids had laminated structure and were composed of mature retinal cell types including cone and rod photoreceptors. Single-cell RNA sequencing was conducted at two time points; this allowed identification of cell types and developmental trajectory characterization of the developing organoids. Important differences between rhesus and human cells were measured regarding the timing and efficiency of retinal organoid differentiation. While the culture of NHP-derived iPSCs is relatively difficult compared to that of human stem cells, the generation of retinal organoids from NHP iPSCs is feasible and may be less time-consuming due to an intrinsically faster timing of retinal differentiation.

CONCLUSIONS: Retinal organoids produced from rhesus monkey iPSCs using established protocols differentiate through the stages of organoid development faster than those derived from human stem cells. The production of NHP retinal organoids may be advantageous to reduce experimental time for basic biology studies in retinogenesis as well as for preclinical trials in NHPs studying retinal allograft transplantation.

Alternate JournalCell Prolif
PubMed ID35165951
PubMed Central IDPMC9055909
Grant ListK08 EY027463 / EY / NEI NIH HHS / United States
K08 EY027463 / GF / NIH HHS / United States
P30 EY012576 / EY / NEI NIH HHS / United States
U24 EY029904 / GF / NIH HHS / United States
R01 EY026942 / EY / NEI NIH HHS / United States
P51 OD011107 / OD / NIH HHS / United States
U24 EY029904 / EY / NEI NIH HHS / United States
/ / Barr Foundation for Retinal Research /

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