Sequence and structure of the extrachromosomal palindrome encoding the ribosomal RNA genes in Dictyostelium.

TitleSequence and structure of the extrachromosomal palindrome encoding the ribosomal RNA genes in Dictyostelium.
Publication TypeJournal Article
Year of Publication2003
AuthorsSucgang, R, Chen, G, Liu, W, Lindsay, R, Lu, J, Muzny, DM, Shaulsky, G, Loomis, W, Gibbs, RA, Kuspa, A
JournalNucleic Acids Res
Date Published2003 May 01
KeywordsAnimals, Chromosome Mapping, Dictyostelium, DNA, Protozoan, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Plasmids, RNA, Ribosomal, Sequence Analysis, DNA

Ribosomal RNAs (rRNAs) are encoded by multicopy families of identical genes. In Dictyostelium and other protists, the rDNA is carried on extrachromosomal palindromic elements that comprise up to 20% of the nuclear DNA. We present the sequence of the 88 kb Dictyostelium rDNA element, noting that the rRNA genes are likely to be the only transcribed regions. By interrogating a library of ordered YAC clones, we provide evidence for a chromosomal copy of the rDNA on chromosome 4. This locus may provide master copies for the stable transmission of the extrachromosomal elements. The extrachromosomal elements were also found to form chromosome-sized clusters of DNA within nuclei of nocodazole-treated cells arrested in mitosis. These clusters resemble true chromosomes and may allow the efficient segregation of the rDNA during mitosis. These rDNA clusters may also explain the cytological observations of a seventh chromosome in this organism.

Alternate JournalNucleic Acids Res
PubMed ID12711681
PubMed Central IDPMC154234
Grant ListR01 HD035925 / HD / NICHD NIH HHS / United States
R01 HD35925 / HD / NICHD NIH HHS / United States

Similar Publications

Wojcik MH, Reuter CM, Marwaha S, Mahmoud M, Duyzend MH, Barseghyan H, et al.. Beyond the exome: What's next in diagnostic testing for Mendelian conditions. Am J Hum Genet. 2023;110(8):1229-1248.
Schlosser P, Zhang J, Liu H, Surapaneni AL, Rhee EP, Arking DE, et al.. Transcriptome- and proteome-wide association studies nominate determinants of kidney function and damage. Genome Biol. 2023;24(1):150.
Lu J, Zheng KQ, Bertrand RElaine, Quinlan J, Ferdous S, Srinivasan T, et al.. Gene augmentation therapy to rescue degenerative photoreceptors in a Cwc27 mutant mouse model. Exp Eye Res. 2023;234:109596.
Calame DG, Guo T, Wang C, Garrett L, Jolly A, Dawood M, et al.. Monoallelic variation in DHX9, the gene encoding the DExH-box helicase DHX9, underlies neurodevelopment disorders and Charcot-Marie-Tooth disease. Am J Hum Genet. 2023;110(8):1394-1413.
Qian X, Srinivasan T, He J, Lu J, Jin Y, Gu H, et al.. Ceramide compensation by ceramide synthases preserves retinal function and structure in a retinal dystrophy mouse model. Dis Model Mech. 2023;16(7).
Yang L, Chen X, Lee C, Shi J, Lawrence EB, Zhang L, et al.. Functional characterization of age-dependent p16 epimutation reveals biological drivers and therapeutic targets for colorectal cancer. J Exp Clin Cancer Res. 2023;42(1):113.
Gao H, Hamp T, Ede J, Schraiber JG, McRae J, Singer-Berk M, et al.. The landscape of tolerated genetic variation in humans and primates. Science. 2023;380(6648):eabn8153.
Sørensen EF, Harris RA, Zhang L, Raveendran M, Kuderna LFK, Walker JA, et al.. Genome-wide coancestry reveals details of ancient and recent male-driven reticulation in baboons. Science. 2023;380(6648):eabn8153.
Shao Y, Zhou L, Li F, Zhao L, Zhang B-L, Shao F, et al.. Phylogenomic analyses provide insights into primate evolution. Science. 2023;380(6648):913-924.
Kuderna LFK, Gao H, Janiak MC, Kuhlwilm M, Orkin JD, Bataillon T, et al.. A global catalog of whole-genome diversity from 233 primate species. Science. 2023;380(6648):906-913.