Sequence variation in the soluble epoxide hydrolase gene and subclinical coronary atherosclerosis: interaction with cigarette smoking.

TitleSequence variation in the soluble epoxide hydrolase gene and subclinical coronary atherosclerosis: interaction with cigarette smoking.
Publication TypeJournal Article
Year of Publication2007
AuthorsWei, Q, Doris, PA, Pollizotto, MV, Boerwinkle, E, Jacobs, DR, Siscovick, DS, Fornage, M
JournalAtherosclerosis
Volume190
Issue1
Pagination26-34
Date Published2007 Jan
ISSN0021-9150
KeywordsAdolescent, Adult, African Americans, Coronary Artery Disease, Epoxide Hydrolases, European Continental Ancestry Group, Female, Gene Frequency, Haplotypes, Humans, Linkage Disequilibrium, Male, Polymorphism, Single Nucleotide, Prospective Studies, Risk Factors, Smoking, Solubility
Abstract

Soluble epoxide hydrolase (EPHX2) catalyses the degradation of the vasoactive and anti-inflammatory epoxyeicosatrienoic acids and may play a role in the pathophysiology of atherosclerosis. 1337 African-Americans and 1645 Whites from the CARDIA study were genotyped for 22 and 15 EPHX2 single nucleotide polymorphisms (SNPs), respectively, to examine the associations of common EPHX2 haplotypes and genotypes with presence of coronary artery calcified plaque (CAC). The potential influence of cigarette smoking, which increases EPHX2 gene expression, on these associations was also assessed. In African-Americans, a common haplotype uniquely tagged by the R287Q polymorphism was associated with significantly greater risk for CAC (OR=1.7; 95% CI=1.04-3.0). In Whites, a common haplotype uniquely tagged by a polymorphism in Intron 11 of the gene was associated with significantly greater risk for CAC (OR=1.3; 95% CI=1.02-1.6). These haplotype-tagging polymorphisms also showed significant associations with CAC in individual SNP analyses, and these relationships were significantly modified by smoking. This detailed investigation of the association of EPHX2 genetic variation with CAC supports EPHX2's emerging role as a risk factor for atherosclerosis, whose effects are influenced by smoking.

DOI10.1016/j.atherosclerosis.2006.02.021
Alternate JournalAtherosclerosis
PubMed ID16545818
Grant ListN01-HC-48047 / HC / NHLBI NIH HHS / United States
N01-HC-48048 / HC / NHLBI NIH HHS / United States
N01-HC-48049 / HC / NHLBI NIH HHS / United States
N01-HC-48050 / HC / NHLBI NIH HHS / United States
N01-HC-95095 / HC / NHLBI NIH HHS / United States
R01-HL69126 / HL / NHLBI NIH HHS / United States
R01-NS41466 / NS / NINDS NIH HHS / United States