Sequencing technologies - the next generation.

TitleSequencing technologies - the next generation.
Publication TypeJournal Article
Year of Publication2010
AuthorsMetzker, ML
JournalNat Rev Genet
Volume11
Issue1
Pagination31-46
Date Published2010 Jan
ISSN1471-0064
KeywordsAutomation, Genetic Techniques, Genome, Genome, Human, Genomics, Humans, Models, Genetic, Molecular Biology, Oligonucleotides, Polymerase Chain Reaction, Sequence Analysis, DNA
Abstract

Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest.

DOI10.1038/nrg2626
Alternate JournalNat. Rev. Genet.
PubMed ID19997069
Grant ListR21 HG002443 / HG / NHGRI NIH HHS / United States
U54 HG003273 / HG / NHGRI NIH HHS / United States
R41 HG003072 / HG / NHGRI NIH HHS / United States
R01 HG003573 / HG / NHGRI NIH HHS / United States
R41 HG003265 / HG / NHGRI NIH HHS / United States