A simple protocol for the automation of DNA cycle sequencing reactions and polymerase chain reactions.

TitleA simple protocol for the automation of DNA cycle sequencing reactions and polymerase chain reactions.
Publication TypeJournal Article
Year of Publication1992
AuthorsCivitello, AB, Richards, S, Gibbs, RA
JournalDNA Seq
Volume3
Issue1
Pagination17-23
Date Published1992
ISSN1042-5179
KeywordsBacteriophage M13, Base Sequence, DNA, Viral, Electrophoresis, Agar Gel, Molecular Sequence Data, Polymerase Chain Reaction, Robotics, Sequence Analysis, DNA
Abstract

Automated DNA sequencing methods using robotic workstations have been previously reported, however it is often an arduous task to import these technologies into a laboratory. We describe protocols making use of a Beckman Biomek 1000 robotic workstation to prepare polymerase chain reactions (PCRs) and "cycle sequencing" reactions to be performed in a Perkin Elmer Cetus System 9600 thermocycler. The combination of these two instruments allows a high throughput of PCR and DNA sequencing reactions. The programs described are freely available via anonymous file transfer protocol (FTP).

Alternate JournalDNA Seq.
PubMed ID1457807
Grant List1-P30HG 0021001 / HG / NHGRI NIH HHS / United States
U01A130243-02 / / PHS HHS / United States