| Title | A simple protocol for the automation of DNA cycle sequencing reactions and polymerase chain reactions. |
| Publication Type | Journal Article |
| Year of Publication | 1992 |
| Authors | Civitello, AB, Richards, S, Gibbs, RA |
| Journal | DNA Seq |
| Volume | 3 |
| Issue | 1 |
| Pagination | 17-23 |
| Date Published | 1992 |
| ISSN | 1042-5179 |
| Keywords | Bacteriophage M13, Base Sequence, DNA, Viral, Electrophoresis, Agar Gel, Molecular Sequence Data, Polymerase Chain Reaction, Robotics, Sequence Analysis, DNA |
| Abstract | Automated DNA sequencing methods using robotic workstations have been previously reported, however it is often an arduous task to import these technologies into a laboratory. We describe protocols making use of a Beckman Biomek 1000 robotic workstation to prepare polymerase chain reactions (PCRs) and "cycle sequencing" reactions to be performed in a Perkin Elmer Cetus System 9600 thermocycler. The combination of these two instruments allows a high throughput of PCR and DNA sequencing reactions. The programs described are freely available via anonymous file transfer protocol (FTP). |
| DOI | 10.3109/10425179209039692 |
| Alternate Journal | DNA Seq |
| PubMed ID | 1457807 |
| Grant List | 1-P30HG 0021001 / HG / NHGRI NIH HHS / United States U01A130243-02 / / PHS HHS / United States |