Targeting iCre expression to murine progesterone receptor cell-lineages using bacterial artificial chromosome transgenesis.

TitleTargeting iCre expression to murine progesterone receptor cell-lineages using bacterial artificial chromosome transgenesis.
Publication TypeJournal Article
Year of Publication2006
AuthorsMukherjee, A, Soyal, SM, Wheeler, DA, Fern√°ndez-Valdivia, R, Nguyen, J, DeMayo, FJ, Lydon, JP
JournalGenesis
Volume44
Issue12
Pagination601-10
Date Published2006 Dec
ISSN1526-954X
KeywordsAnimals, Cell Lineage, Chromosomes, Artificial, Bacterial, Computational Biology, DNA Primers, Gene Components, Gene Transfer Techniques, Integrases, Mice, Mice, Transgenic, Models, Animal, Promoter Regions, Genetic, Receptors, Progesterone
Abstract

Gene-targeting in embryonic stem cells has been the dominant genetic approach when engineering mouse models to query the physiologic importance of the progesterone receptor (PR). Although these models have been instrumental in disclosing the in vivo significance of the progesterone signaling pathway, generation of such mice exacts considerable expenditure of time, effort, and expense. Considering the growing list of new PR mouse models that are urgently required to address the next questions in progestin biology, bacterial artificial chromosome (BAC) recombineering in conjunction with transgenesis was evaluated as an alternative method to accelerate the creation of these models in the future. Using this approach, we describe the generation of three PR-BAC(iCre) transgenic lines in which improved Cre recombinase (iCre) was targeted in-frame, downstream, and under the control of the PR promoter contained within a BAC transgene. Crossing with the ROSA26R revealed that the PR-BAC(iCre) transgenic expresses active iCre only in cell-lineages that express the PR. The specificity of the PR-BAC(iCre) transgene not only underscores the importance of BAC-mediated transgenesis as a quick, easy, and affordable method by which to engineer the next generation of PR mouse models, but also provides a unique opportunity to investigate transcriptional control of PR expression as well as PR structure-function relationships in vivo.

DOI10.1002/dvg.20257
Alternate JournalGenesis
PubMed ID17149722
Grant ListCA077530 / CA / NCI NIH HHS / United States
HD42311 / HD / NICHD NIH HHS / United States