Technical note: multi-alleles at the DYS385ab locus with high frequency in a Han Chinese population from southwestern China.

TitleTechnical note: multi-alleles at the DYS385ab locus with high frequency in a Han Chinese population from southwestern China.
Publication TypeJournal Article
Year of Publication2021
AuthorsFu, J, Fu, S, Yin, S, Cheng, J, Liu, X, Jin, Z, He, T, Fu, J
JournalInt J Legal Med
Volume135
Issue5
Pagination1737-1741
Date Published2021 Sep
ISSN1437-1596
KeywordsAlleles, Asian People, China, Chromosomes, Human, Y, Gene Frequency, Genetic Loci, Genotype, Humans, Male, Microsatellite Repeats
Abstract

Y-chromosome short tandem repeat (Y-STR) markers have been widely used in forensic applications and usually show monoallelic or diallelic genotypic patterns at certain double-copied loci. In this study, we have found 13 samples among 703 males with multi-alleles at the DYS385ab locus, including one with five mutant alleles, nine with four, and three with three. The frequency of abnormal DYS385ab genotypes was 1.85% (13/703), which is very high in the Han Chinese population. The percentage of samples with diallelic patterns at DYS385ab was higher than that of monoallelic patterns (80.23% vs. 17.92%). Additionally, the percentage of samples with tetra-allelic patterns at DYS385ab was higher than that of tri-allelic patterns (1.28% vs. 0.43%), suggesting that there are possibly two copies with duplicated events happening frequently on the Y chromosome. Interestingly, the peak height of allele 13 was two to three-folds higher than that of other alleles. The allele 18 peak height was also two-fold higher than others, which could potentially be explained by a duplication event mechanism. We also found that tri-allelic genotypes for alleles 13, 17, and 20, tetra-allelic genotypes for alleles 13, 14, 19, and 20, and tetra-allelic genotypes for alleles 12, 13, 19 and 21 were more common than others. Furthermore, all 13 samples had multi-alleles containing allele 13, implying a founder effect in this particular Chinese-specific ethnic group. Taken together, this study provides new information for this population and will be useful for paternal lineage identification, kinship analysis, and family relationship reconstruction using Y-STR forensic DNA analysis methods.

DOI10.1007/s00414-021-02592-1
Alternate JournalInt J Legal Med
PubMed ID33844081
PubMed Central ID1918216
Grant List81672887 / / National Natural Science Foundation of China /

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