Transcriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice.

TitleTranscriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice.
Publication TypeJournal Article
Year of Publication2019
AuthorsPark, YH, Snook, JD, Ostrin, EJ, Kim, S, Chen, R, Frankfort, BJ
JournalSci Rep
Volume9
Issue1
Pagination2594
Date Published2019 Feb 22
ISSN2045-2322
Abstract

Elevated intraocular pressure (IOP) is the major risk factor for glaucoma, a sight threatening disease of retinal ganglion cells (RGCs) and their axons. Despite the central importance of IOP, details of the impact of IOP elevation on RGC gene expression remain elusive. We developed a 4-step immunopanning protocol to extract adult mouse RGCs with high fidelity and used it to isolate RGCs from wild type mice exposed to 2 weeks of IOP elevation generated by the microbead model. IOP was elevated to 2 distinct levels which were defined as Mild (IOP increase >1 mmHg and

DOI10.1038/s41598-019-39141-1
Alternate JournalSci Rep
PubMed ID30796289
PubMed Central IDPMC6385489
Grant ListP30 EY002520 / EY / NEI NIH HHS / United States
S10 OD023469 / OD / NIH HHS / United States
P30EY002520 / / U.S. Department of Health & Human Services | NIH | National Eye Institute (NEI) /
S10OD023469 / / U.S. Department of Health & Human Services | NIH | NIH Office of the Director (OD) /