TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development.

TitleTREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development.
Publication TypeJournal Article
Year of Publication2002
AuthorsGingras, M-C, Lapillonne, H, Margolin, JF
JournalMol Immunol
Date Published2002 Mar
KeywordsAdaptor Proteins, Signal Transducing, Amino Acid Sequence, Base Sequence, Cell Differentiation, Hematopoietic Stem Cells, Humans, Lectins, C-Type, Leukemia, Membrane Glycoproteins, Membrane Proteins, Molecular Sequence Data, Monocytes, Receptors, Cell Surface, Receptors, Immunologic, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Triggering Receptor Expressed on Myeloid Cells-1

The triggering receptor expressed on myeloid cells (TREM-1) and the myeloid DAP12-associating lectin (MDL-1) are two recently identified receptors which associate non-covalently with DAP12 to form receptor complexes involved in monocytic activation and inflammatory response. In this study, we investigated whether the expression of TREM-1, MDL-1, and DAP12 correlated with myelomonocytic differentiation. Northern and RT-PCR revealed a strong expression of TREM-1, MDL-1, and DAP12 in peripheral blood-derived CD14(+) mature monocytes in contrast to undifferentiated bone marrow CD34(+) stem cells, and in the differentiated versus undifferentiated U937 cells. TREM-1 and MDL-1 RNA expression was also more elevated in adult than fetal tissues and in normal than malignant cells. These findings suggest that the TREM-1/DAP12 and MDL-1/DAP12 signaling pathways are features of mature differentiated myelomonocytic cells. In addition, expression of an alternative mRNA TREM-1 splice variant (TREM-1sv) was detected that might translate into a soluble receptor with potential as a regulator of myeloid activation.

Alternate JournalMol Immunol
PubMed ID11922939
Grant ListU01 CA080200 / CA / NCI NIH HHS / United States

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