Whole exome capture in solution with 3 Gbp of data.

Title	Whole exome capture in solution with 3 Gbp of data.
Publication Type	Journal Article
Year of Publication	2010
Authors	Bainbridge, MN, Wang, M, Burgess, DL, Kovar, C, Rodesch, MJ, D'Ascenzo, M, Kitzman, J, Wu, Y-Q, Newsham, I, Richmond, TA, Jeddeloh, JA, Muzny, DM, Albert, TJ, Gibbs, RA
Journal	Genome Biol
Volume	11
Issue	6
Pagination	R62
Date Published	2010
ISSN	1474-760X
Keywords	Base Pairing, Databases, Nucleic Acid, Exons, Gene Library, Haplotypes, Humans, Polymorphism, Single Nucleotide, Reproducibility of Results, Sequence Alignment, Sequence Analysis, DNA, Solutions
Abstract	We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies.
DOI	10.1186/gb-2010-11-6-r62
Alternate Journal	Genome Biol
PubMed ID	20565776
PubMed Central ID	PMC2911110
Grant List	U54 HG003273 / HG / NHGRI NIH HHS / United States U54HG003273 / HG / NHGRI NIH HHS / United States