Whole-Exome Sequencing Identifies Loci Associated with Blood Cell Traits and Reveals a Role for Alternative GFI1B Splice Variants in Human Hematopoiesis.

TitleWhole-Exome Sequencing Identifies Loci Associated with Blood Cell Traits and Reveals a Role for Alternative GFI1B Splice Variants in Human Hematopoiesis.
Publication TypeJournal Article
Year of Publication2016
AuthorsPolfus, LM, Khajuria, RK, Schick, UM, Pankratz, N, Pazoki, R, Brody, JA, Chen, M-H, Auer, PL, Floyd, JS, Huang, J, Lange, L, van Rooij, FJA, Gibbs, RA, Metcalf, G, Muzny, DM, Veeraraghavan, N, Walter, K, Chen, L, Yanek, L, Becker, LC, Peloso, GM, Wakabayashi, A, Kals, M, Metspalu, A, Esko, T, Fox, K, Wallace, R, Franceshini, N, Matijevic, N, Rice, KM, Bartz, TM, Lyytikäinen, L-P, Kähönen, M, Lehtimäki, T, Raitakari, OT, Li-Gao, R, Mook-Kanamori, DO, Lettre, G, van Duijn, CM, Franco, OH, Rich, SS, Rivadeneira, F, Hofman, A, Uitterlinden, AG, Wilson, JG, Psaty, BM, Soranzo, N, Dehghan, A, Boerwinkle, E, Zhang, X, Johnson, AD, O'Donnell, CJ, Johnsen, JM, Reiner, AP, Ganesh, SK, Sankaran, VG
JournalAm J Hum Genet
Volume99
Issue2
Pagination481-8
Date Published2016 Aug 04
ISSN1537-6605
Abstract

Circulating blood cell counts and indices are important indicators of hematopoietic function and a number of clinical parameters, such as blood oxygen-carrying capacity, inflammation, and hemostasis. By performing whole-exome sequence association analyses of hematologic quantitative traits in 15,459 community-dwelling individuals, followed by in silico replication in up to 52,024 independent samples, we identified two previously undescribed coding variants associated with lower platelet count: a common missense variant in CPS1 (rs1047891, MAF = 0.33, discovery + replication p = 6.38 × 10(-10)) and a rare synonymous variant in GFI1B (rs150813342, MAF = 0.009, discovery + replication p = 1.79 × 10(-27)). By performing CRISPR/Cas9 genome editing in hematopoietic cell lines and follow-up targeted knockdown experiments in primary human hematopoietic stem and progenitor cells, we demonstrate an alternative splicing mechanism by which the GFI1B rs150813342 variant suppresses formation of a GFI1B isoform that preferentially promotes megakaryocyte differentiation and platelet production. These results demonstrate how unbiased studies of natural variation in blood cell traits can provide insight into the regulation of human hematopoiesis.

DOI10.1016/j.ajhg.2016.06.016
Alternate JournalAm. J. Hum. Genet.
PubMed ID27486782
PubMed Central IDPMC4974169
Grant ListR01 DK103794 / DK / NIDDK NIH HHS / United States
R01 HL122684 / HL / NHLBI NIH HHS / United States
R21 HL120791 / HL / NHLBI NIH HHS / United States
R33 HL120791 / HL / NHLBI NIH HHS / United States