The Baylor College of Medicine Human Genome Sequencing Center (Baylor-HGSC) is one of three large-scale sequencing centers funded by the National Institutes of Health. As one of the three major centers in the United States, the Baylor-HGSC provides a unique opportunity to work with cutting edge genomic science. The Human Genome Sequencing Center (HGSC) at Baylor College of Medicine is seeking to recruit a Research Assistant (RA- I) to work in the Library Construction group. The candidate should be able to work independently and within a team to process samples in the production pipeline. He/she should be flexible and adapt to the pipeline requirements and complete assigned tasks in a timely manner. Qualified candidates should be good at multi-tasking, problem-solving, and have strong communication skills. The Library Construction group is responsible for preparation of samples for sequencing across multiple NextGen sequencing platforms. The position would principally involve in sample preparation for next generation sequencing (NGS) across multiple pipelines including DNA QC, construction of DNA and RNA libraries for NGS DNA libraries for Next-Generation sequencing pipelines (Illumina, PacBio and others) and target enrichment (capture) sequencing. His/her essential functions and duties include processing samples with Beckman Robots for lab procedures including library construction, qPCR, hybridization for Next Generation Sequencing (NGS). Work will also include maintenance of instruments such as Covaris Sonicator, Beckman Robots, Fragment Analyzer, documents and preliminary interpretation of test results and basic troubleshooting.
- Work independently and within a team to process samples in the production pipeline, as well as work on R&D projects including protocol development and optimization.
- Required to supervise and train others to plan and conducting experiments, and work under the direction of the team leader and the group manager.
- Perform laboratory research projects and production tasks.
- Update LIMS, sample tracking and summarize data in an appropriate format and report to the team leader.
- Troubleshoot problematic results
- Perform routine laboratory equipment maintenance, reagent tracking and validation, reagent ordering and log records as required.
- Oversee the maintenance of lab equipment and environment
- Master's degree in a basic science or equivalent*.
- *NOTE: A Bachelor's degree in a basic science and two years of directly related experience is considered equivalent to a Master's degree.
- No experience required
- Qualified candidates should have strong molecular biology experience, good organizational and communication skills and be a quick learner.
- Expertise in one or more of the following molecular biology areas is essential: DNA and RNA manipulations such as purification and qualification, PCR, qPCR and restriction enzyme digestion is preferred
- Prior experience in Next Generation sequencing library construction, qPCR, Illumina arrays and automated liquid handling.