Skip to Content

Large-scale sequencing library production: an adaptor-based strategy.

TitleLarge-scale sequencing library production: an adaptor-based strategy.
Publication TypeJournal Article
Year of Publication1993
AuthorsPovinelli, C. M., and Gibbs R. A.
JournalAnalytical biochemistry
Date Published1993 Apr
KeywordsAntigens, CD4, Base Sequence, Biotechnology, Cloning, Molecular, DNA, Evaluation Studies as Topic, Gene Library, Genetic Techniques, Genetic Vectors, Humans, Molecular Sequence Data, Sequence Analysis, DNA, Software Design
AbstractA significant bottleneck in large-scale DNA sequencing projects is the creation of high-quality random libraries suitable for sequencing. We have utilized an adaptor-based strategy and developed a simple protocol to reliably generate single-insert random subclone M13 libraries yielding uniform coverage and > 10(3) clones per microgram of DNA. Of the resulting white plaques, 95% contained inserts, < 0.5% were due to adaptors with no insert, and none of the clones were found to contain extraneous DNA (0/600) or multiple inserts (0/300).
Alternate JournalAnal. Biochem.

about seo | biblio