| Title | Sequencing and functional analysis of the SNRPN promoter: in vitro methylation abolishes promoter activity. |
| Publication Type | Journal Article |
| Year of Publication | 1997 |
| Authors | Huq, AH, Sutcliffe, JS, Nakao, M, Shen, Y, Gibbs, RA, Beaudet, AL |
| Journal | Genome Res |
| Volume | 7 |
| Issue | 6 |
| Pagination | 642-8 |
| Date Published | 1997 Jun |
| ISSN | 1088-9051 |
| Keywords | Alleles, Autoantigens, Cells, Cultured, Chromosomes, Human, Pair 15, Cloning, Molecular, CpG Islands, DNA Methylation, DNA-Cytosine Methylases, Exons, Gene Expression, HeLa Cells, Humans, Methylation, Molecular Sequence Data, Polymerase Chain Reaction, Prader-Willi Syndrome, Promoter Regions, Genetic, Repetitive Sequences, Nucleic Acid, Ribonucleoproteins, Small Nuclear, Sequence Analysis, DNA, snRNP Core Proteins, Transfection |
| Abstract | The gene encoding the small nuclear ribonucleoprotein-associated polypeptide N (SNRPN) maps to the Prader-Willi syndrome critical region on chromosome 15 and is expressed preferentially from the paternal allele. A CpG island encompassing the first exon of SNRPN is methylated on the inactive maternal allele. DNA sequence was determined for a cosmid containing the first three exons of SNRPN and extending 20 kb upstream and 15 kb downstream from the CpG island. This region is extremely rich in Alu elements and other repetitive sequences and contains a single CpG island, which includes numerous short direct repeat sequences. Functional analysis of the first exon revealed strong promoter activity for a 260-bp fragment extending 207 bp upstream from the exon. In vitro methylation of this 260-bp fragment abolished promoter activity completely, suggesting that the silencing of the maternal SNRPN allele may be a direct consequence of methylation of the promoter region.
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| DOI | 10.1101/gr.7.6.642 |
| Alternate Journal | Genome Res |
| PubMed ID | 9199937 |
| PubMed Central ID | PMC310659 |
| Grant List | R01 HG01459 / HG / NHGRI NIH HHS / United States |