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Comprehensive molecular characterization of human colon and rectal cancer.

TitleComprehensive molecular characterization of human colon and rectal cancer.
Publication TypeJournal Article
Year of Publication2012
AuthorsShinbrot, Eve
Corporate AuthorsCancer Genome Atlas Network
Date Published2012 Jul 19
KeywordsColonic Neoplasms, DNA Copy Number Variations, DNA Methylation, Exome, Gene Expression Profiling, Humans, Mutation, Mutation Rate, Polymorphism, Single Nucleotide, Rectal Neoplasms, Sequence Analysis, DNA
AbstractTo characterize somatic alterations in colorectal carcinoma, we conducted a genome-scale analysis of 276 samples, analysing exome sequence, DNA copy number, promoter methylation and messenger RNA and microRNA expression. A subset of these samples (97) underwent low-depth-of-coverage whole-genome sequencing. In total, 16% of colorectal carcinomas were found to be hypermutated: three-quarters of these had the expected high microsatellite instability, usually with hypermethylation and MLH1 silencing, and one-quarter had somatic mismatch-repair gene and polymerase ε (POLE) mutations. Excluding the hypermutated cancers, colon and rectum cancers were found to have considerably similar patterns of genomic alteration. Twenty-four genes were significantly mutated, and in addition to the expected APC, TP53, SMAD4, PIK3CA and KRAS mutations, we found frequent mutations in ARID1A, SOX9 and FAM123B. Recurrent copy-number alterations include potentially drug-targetable amplifications of ERBB2 and newly discovered amplification of IGF2. Recurrent chromosomal translocations include the fusion of NAV2 and WNT pathway member TCF7L1. Integrative analyses suggest new markers for aggressive colorectal carcinoma and an important role for MYC-directed transcriptional activation and repression.
Alternate JournalNature

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