The Baylor College of Medicine Human Genome Sequencing Center (BCM-HGSC) team is offering long read sequencing to those interested. Please see sample quantity and quality requirements below.
DNA quality is critical to performance and final results.
The BCM-HGSC is offering the below applications for each platform:
Instrument: Pacific Biosciences Sequel II
| Applications | Minimum DNA (by Qubit) | QC Requirements |
|---|---|---|
| Whole Genome Sequencing - CLR | 3-5 ug | >50 Kb; 260/280 >1.8; 260/230>2.0 |
| Whole Genome Sequencing - HiFi | 10-15 ug | |
| Amplicon (1-20 Kb) | 100 ng/amplicon | |
| Whole Genome Sequencing - Microbial | 1 ug/sample | >80% gDNA >20 kb; 260/280 >1.8; 260/230>2.0 |
| 16S | 0.05 ng - 5 ng/sample | 260/280 >1.8; 260/230>2.0 |
| RNA Iso-Seq | 500 ng - 1 ug | RIN≥7.0 |
Instrument: Oxford Nanopore PromethION
| Applications | Minimum DNA (by Qubit) | QC Requirements |
|---|---|---|
| Whole Genome Sequencing | 3 ug for insert size 15 kb~20 kb; more for larger insert size | >50 Kb; 260/280 >1.8; 260/230>2.0 |
**DNA or RNA should be suspended in Qiagen Elution Buffer, 10 mM Tris-Cl, pH 8.5. If needed, we can perform DNA and/or RNA Isolation for blood or tissue samples.
Deliverables: There will be a one-time data transfer for a given project including raw and/or mapped data. Mapped data will only be available for human samples and if a version is provided during sample submission.
Timeline: Project dependent. We will provide an estimate after your sequencing request is submitted.
Keep in mind – the cost of sequencing is reduced when sequencing multiple SMRT/flowcells.
If you are interested please fill out the form below.