| Title | Direct selection of human genomic loci by microarray hybridization. |
| Publication Type | Journal Article |
| Year of Publication | 2007 |
| Authors | Albert, TJ, Molla, MN, Muzny, DM, Nazareth, L, Wheeler, DA, Song, X, Richmond, TA, Middle, CM, Rodesch, MJ, Packard, CJ, Weinstock, GM, Gibbs, RA |
| Journal | Nat Methods |
| Volume | 4 |
| Issue | 11 |
| Pagination | 903-5 |
| Date Published | 2007 Nov |
| ISSN | 1548-7091 |
| Keywords | BRCA1 Protein, Cell Line, Tumor, Gene Library, Genome, Human, Humans, Nucleic Acid Hybridization, Oligodeoxyribonucleotides, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Sequence Analysis, DNA |
| Abstract | We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions. |
| DOI | 10.1038/nmeth1111 |
| Alternate Journal | Nat Methods |
| PubMed ID | 17934467 |
| Grant List | R21HG004553 / HG / NHGRI NIH HHS / United States U54 HG 003273 / HG / NHGRI NIH HHS / United States |