Direct selection of human genomic loci by microarray hybridization.

TitleDirect selection of human genomic loci by microarray hybridization.
Publication TypeJournal Article
Year of Publication2007
AuthorsAlbert, TJ, Molla, MN, Muzny, DM, Nazareth, L, Wheeler, DA, Song, X, Richmond, TA, Middle, CM, Rodesch, MJ, Packard, CJ, Weinstock, GM, Gibbs, RA
JournalNat Methods
Volume4
Issue11
Pagination903-5
Date Published2007 Nov
ISSN1548-7091
KeywordsBRCA1 Protein, Cell Line, Tumor, Gene Library, Genome, Human, Humans, Nucleic Acid Hybridization, Oligodeoxyribonucleotides, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Sequence Analysis, DNA
Abstract

We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.

DOI10.1038/nmeth1111
Alternate JournalNat. Methods
PubMed ID17934467
Grant ListR21HG004553 / HG / NHGRI NIH HHS / United States
U54 HG 003273 / HG / NHGRI NIH HHS / United States