Emerging technologies in DNA sequencing.

 
TitleEmerging technologies in DNA sequencing.
Publication TypeJournal Article
Year of Publication2005
AuthorsMetzker, ML
JournalGenome Res
Volume15
Issue12
Pagination1767-76
Date Published2005 Dec
ISSN1088-9051
KeywordsAnimals, Base Sequence, DNA, DNA-Directed DNA Polymerase, Humans, Sequence Analysis, DNA
Abstract

Demand for DNA sequence information has never been greater, yet current Sanger technology is too costly, time consuming, and labor intensive to meet this ongoing demand. Applications span numerous research interests, including sequence variation studies, comparative genomics and evolution, forensics, and diagnostic and applied therapeutics. Several emerging technologies show promise of delivering next-generation solutions for fast and affordable genome sequencing. In this review article, the DNA polymerase-dependent strategies of Sanger sequencing, single nucleotide addition, and cyclic reversible termination are discussed to highlight recent advances and potential challenges these technologies face in their development for ultrafast DNA sequencing.

DOI10.1101/gr.3770505
Alternate JournalGenome Res.
PubMed ID16339375
Grant ListR01 HG003573 / HG / NHGRI NIH HHS / United States
R21 HG002443 / HG / NHGRI NIH HHS / United States
R41 HG003072 / HG / NHGRI NIH HHS / United States
R41 HG003265 / HG / NHGRI NIH HHS / United States