| Title | Emerging technologies in DNA sequencing. |
| Publication Type | Journal Article |
| Year of Publication | 2005 |
| Authors | Metzker, ML |
| Journal | Genome Res |
| Volume | 15 |
| Issue | 12 |
| Pagination | 1767-76 |
| Date Published | 2005 Dec |
| ISSN | 1088-9051 |
| Keywords | Animals, Base Sequence, DNA, DNA-Directed DNA Polymerase, Humans, Sequence Analysis, DNA |
| Abstract | Demand for DNA sequence information has never been greater, yet current Sanger technology is too costly, time consuming, and labor intensive to meet this ongoing demand. Applications span numerous research interests, including sequence variation studies, comparative genomics and evolution, forensics, and diagnostic and applied therapeutics. Several emerging technologies show promise of delivering next-generation solutions for fast and affordable genome sequencing. In this review article, the DNA polymerase-dependent strategies of Sanger sequencing, single nucleotide addition, and cyclic reversible termination are discussed to highlight recent advances and potential challenges these technologies face in their development for ultrafast DNA sequencing. |
| DOI | 10.1101/gr.3770505 |
| Alternate Journal | Genome Res |
| PubMed ID | 16339375 |
| Grant List | R01 HG003573 / HG / NHGRI NIH HHS / United States R21 HG002443 / HG / NHGRI NIH HHS / United States R41 HG003072 / HG / NHGRI NIH HHS / United States R41 HG003265 / HG / NHGRI NIH HHS / United States |