A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum.

TitleA full-length cDNA resource for the pea aphid, Acyrthosiphon pisum.
Publication TypeJournal Article
Year of Publication2010
AuthorsShigenobu, S, Richards, S, Cree, AG, Morioka, M, Fukatsu, T, Kudo, T, Miyagishima, S, Gibbs, RA, Stern, DL, Nakabachi, A
JournalInsect Mol Biol
Volume19 Suppl 2
Pagination23-31
Date Published2010 Mar
ISSN1365-2583
Keywords3' Untranslated Regions, 5' Untranslated Regions, Animals, Aphids, Base Sequence, DNA Primers, DNA, Complementary, Expressed Sequence Tags, Gene Library, Genome, Insect, Host-Pathogen Interactions, Models, Genetic, Peas
Abstract

Large collections of full-length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full-length cDNA clones from the pea aphid, Acyrthosiphon pisum. Sequencing from 5' and 3' ends of the clones generated 97 828 high-quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full-length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full-length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.

DOI10.1111/j.1365-2583.2009.00946.x
Alternate JournalInsect Mol. Biol.
PubMed ID20482637
PubMed Central IDPMC4370113
Grant ListU54 HG003273 / HG / NHGRI NIH HHS / United States