A BAC-based physical map of the major autosomes of Drosophila melanogaster.

TitleA BAC-based physical map of the major autosomes of Drosophila melanogaster.
Publication TypeJournal Article
Year of Publication2000
AuthorsHoskins, RA, Nelson, CR, Berman, BP, Laverty, TR, George, RA, Ciesiolka, L, Naeemuddin, M, Arenson, AD, Durbin, J, David, RG, Tabor, PE, Bailey, MR, DeShazo, DR, Catanese, J, Mammoser, A, Osoegawa, K, de Jong, PJ, Celniker, SE, Gibbs, RA, Rubin, GM, Scherer, SE
JournalScience
Volume287
Issue5461
Pagination2271-4
Date Published2000 Mar 24
ISSN0036-8075
KeywordsAnimals, Centromere, Chromatin, Chromosomes, Bacterial, Cloning, Molecular, Contig Mapping, DNA Fingerprinting, Drosophila melanogaster, Euchromatin, Gene Library, Genes, Insect, Genetic Markers, Genetic Vectors, Genome, In Situ Hybridization, Repetitive Sequences, Nucleic Acid, Restriction Mapping, Sequence Analysis, DNA, Sequence Tagged Sites, Telomere
Abstract

We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

Alternate JournalScience
PubMed ID10731150
Grant ListHG00750 / HG / NHGRI NIH HHS / United States